pcmvha he2f1 vector  (Addgene inc)

Journal: Clinical epigenetics

Article Title: E2F1 combined with LINC01004 super-enhancer to promote hepatocellular carcinoma cell proliferation and metastasis.

doi: 10.1186/s13148-023-01428-6

Figure Lengend Snippet: Fig. 4 E2F1 combined with LINC01004 SE to promote its expression. A The IGV results of H3K27ac ChIP-seq, E2F1 ChIP-seq, and ATAC-seq. B E2F1 ChIP-qPCR results of HepG2 cells. IgG antibody was used as negative control. C Correlation analysis of E2F1 and LINC01004 in tumor tissues of TCGA HCC patients. D qRT-PCR results of E2F1 and LINC01004 in HepG2 cells. E Western blot results of E2F1. F Schematic diagram of pGL3-enhancer vectors. G Dual-luciferase reporter system assay of pGL3-enhancer vector. pGL3-basic vector was used as negative control

Article Snippet: Cells were plated and grown until they were reached 70–80% confluent. pCMVHA E2F1 was a gift from Kristian Helin [23] (Addgene plasmid # 24,225, https:// www. addge ne. org/ 24225/, RRID:Addgene_24225). pLV hUbCdCas9 KRAB-T2A-GFP was a gift from Charles Gersbach (Addgene plasmid # 67,620, https:// www. addge ne. org/ 67620/, RRID:Addgene_67620) [24].

Techniques: Expressing, ChIP-sequencing, ChIP-qPCR, Negative Control, Quantitative RT-PCR, Western Blot, Luciferase, Plasmid Preparation